Brand  (β version)

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Ligands
Code Name Link
2Z0 N-{[5-(dimethylamino)naphthalen-2-yl]sulfonyl}-L-alpha-glutamyl-N-[(1s)-4-{[amino(iminio)methyl]amino}-1-(chloroacetyl)butyl]glycinamide
Code : 1BDA
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : CATALYTIC DOMAIN OF HUMAN SINGLE CHAIN TISSUE PLASMINOGEN ACTIVATOR IN COMPLEX WITH DANSYL-EGR-CMK (DANSYL-GLU-GLY-ARG CHLOROMETHYL KETONE)
Release Data : 1999-05-11
Compound :
mol_id molecule chains synonym
1 SINGLE CHAIN TISSUE TYPE PLASMINOGEN ACTIVATOR A,B SC-TPA
ec: 3.4.21.68
fragment: UNP residues 298-562
mutation: DELETION MUTANT
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
Authors : Bode, W., Renatus, M., Engh, R.A.
Keywords : TRYPSIN LIKE SERINE PROTEASE, FIBRINOLYTIC ENZYMES, PLASMINOGEN ACTIVATORS, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
Exp. method : X-RAY DIFFRACTION ( 3.35 Å )
Citation :

Lysine 156 promotes the anomalous proenzyme activity of tPA: X-ray crystal structure of single-chain human tPA.

Renatus, M.,Engh, R.A.,Stubbs, M.T.  et al.
(1997)  EMBO J.  16 : 4797 - 4805

PubMed: 9305622
DOI: 10.1093/emboj/16.16.4797

Catalytic Domain Structure of Vampire Bat Plasminogen Activator: A Molecular Paradigm for Proteolysis without Activation Cleavage

Renatus, M.,Stubbs, M.T.,Huber, R.  et al.
(1997)  Biochemistry  36 : 13483

The 2.3 A Crystal Structure of the Catalytic Domain of Recombinant Two-Chain Human Tissue-Type Plasminogen Activator

Lamba, D.,Bauer, M.,Huber, R.  et al.
(1996)  J.Mol.Biol.  258 : 117

Chain : A, B
UniProt : P00750 (TPA_HUMAN)
Reaction : Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.