Brand  (β version)

PDB ID: 1B9X

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Ligands
Code Name Style Show Link
GD Gadolinium atom PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1B9X   PDBj   RCSB PDB   PDBe
Header : SIGNALING PROTEIN
Title : STRUCTURAL ANALYSIS OF PHOSDUCIN AND ITS PHOSPHORYLATION-REGULATED INTERACTION WITH TRANSDUCIN
Release Data : 1999-02-23
Compound :
mol_id molecule chains synonym
1 PROTEIN (TRANSDUCIN) A GT BETA
fragment: LYS-C RESISTANT FRAGMENT, THE BETA SUBUNIT
mol_id molecule chains synonym
2 PROTEIN (TRANSDUCIN) B GT GAMMA
fragment: LYS-C RESISTANT FRAGMENT, THE GAMMA SUBUNIT CLEAVED AFTER RESIDUE 68
mol_id molecule chains synonym
3 PROTEIN (PHOSDUCIN) C MEKA, PP33
mutation: S73E
Source :
mol_id organism_scientific organism_common
1 Bos taurus  (taxid:9913) Cattle
organ: EYE
tissue: RETINA
cellular_location: ROD OUTER SEGMENTS
other_details: PURIFIED FROM BOVINE ROD OUTER SEGMENTS
mol_id organism_scientific organism_common
2 Bos taurus  (taxid:9913) Cattle
organ: EYE
tissue: RETINA
cellular_location: ROD OUTER SEGMENTS
other_details: PURIFIED FROM BOVINE ROD OUTER SEGMENTS
mol_id organism_scientific organism_common expression_system
3 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli BL21(DE3)  (taxid:469008)
organ: PINEAL GLAND, RETINA
cellular_location: CYTOSOLIC
gene: RAT PDC
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_vector: PET15B
expression_system_plasmid: PET15B/S73E
expression_system_gene: RAT PDC
other_details: N-TERMINAL EXTENSION OF THE SEQUENCE MGSSHHHHHHSSGLVPRGSH.
Authors : Gaudet, R., Sigler, P.B.
Keywords : PHOSDUCIN, TRANSDUCIN, BETA-GAMMA, SIGNAL TRANSDUCTION, REGULATION, PHOSPHORYLATION, G PROTEINS, THIOREDOXIN, VISION, MEKA, COMPLEX (TRANSDUCER- TRANSDUCTION), SIGNALING PROTEIN
Exp. method : X-RAY DIFFRACTION ( 3.0 Å )
Citation :

A molecular mechanism for the phosphorylation-dependent regulation of heterotrimeric G proteins by phosducin.

Gaudet, R.,Savage, J.R.,McLaughlin, J.N.  et al.
(1999)  Mol.Cell  3 : 649 - 660

PubMed: 10360181
DOI: 10.1016/S1097-2765(00)80358-5

Crystal structure of a G-protein beta gamma dimer at 2.1A resolution.

Sondek, J.,Bohm, A.,Lambright, D.G.  et al.
(1996)  Nature  379 : 369 - 374

PubMed: 8552196
DOI: 10.1038/379369a0

Reaction

Chain : A
UniProt : P62871 (GBB1_BOVIN)
Reaction : -
Chain : B
UniProt : P02698 (GBG1_BOVIN)
Reaction : -
Chain : C
UniProt : P20942 (PHOS_RAT)
Reaction : -