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Ligands
Code Name Style Show Link
CAC Cacodylate ion
SO4 Sulfate ion
Non-standard Residues
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Glycosylation
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Code : 1B9D   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : MOBILITY OF AN HIV-1 INTEGRASE ACTIVE SITE LOOP IS CORRELATED WITH CATALYTIC ACTIVITY
Release Data : 1999-07-19
Compound :
mol_id molecule chains
1 PROTEIN (INTEGRASE) A
ec: 2.7.7.49
fragment: CATALYTIC CORE DOMAIN
mutation: F185K
Source :
mol_id organism_scientific expression_system
1 Human immunodeficiency virus  (taxid:11676) Escherichia coli BL21(DE3)  (taxid:469008)
strain: 1
expression_system_strain: BL21
expression_system_variant: DE3
expression_system_cellular_location: CYTOPLASM
expression_system_vector: PET28
Authors : Greenwald, J., Le, V., Butler, S.L., Bushman, F.D., Choe, S.
Keywords : DNA INTEGRATION, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.7 Å )
Citation :

The mobility of an HIV-1 integrase active site loop is correlated with catalytic activity.

Greenwald, J.,Le, V.,Butler, S.L.  et al.
(1999)  Biochemistry  38 : 8892 - 8898

PubMed: 10413462
DOI: 10.1021/bi9907173

Chain : A
UniProt : P12497 (POL_HV1N5)
Reaction: EC: Evidence:
Physiological Direction:
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro. 3.4.23.16 PROSITE-ProRule:PRU00275
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Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. 3.1.26.13 ECO:0000250
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3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. 3.1.13.2 ECO:0000250
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a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.49 PROSITE- ProRule:PRU00405
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a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 PROSITE- ProRule:PRU00405
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