PDB ID: 1B9D
Hetero Atom Contents
color scheme of protein:
|Title :||MOBILITY OF AN HIV-1 INTEGRASE ACTIVE SITE LOOP IS CORRELATED WITH CATALYTIC ACTIVITY|
|Release Data :||1999-07-19|
|Authors :||Greenwald, J., Le, V., Butler, S.L., Bushman, F.D., Choe, S.|
|Keywords :||DNA INTEGRATION, TRANSFERASE|
|Exp. method :||X-RAY DIFFRACTION ( 1.7 Å )|
|UniProt :||P12497 (POL_HV1N5)|
|Reaction :||Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).