Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
GOL Glycerol
SO4 Sulfate ion
Modified Residues
Code Name Link
MDO {2-[(1s)-1-aminoethyl]-4-methylidene-5-oxo-4,5-dihydro-1h-imidazol-1-yl}acetic acid
Code : 1B8F
Header : LYASE
Title : Histidine ammonia-lyase (HAL) from Pseudomonas putida
Release Data : 1999-05-06
Compound :
mol_id molecule chains synonym
1 Histidine ammonia-lyase A HAL, Histidase
ec: 4.3.1.3
mutation: C273A
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:469008)
atcc: 12633
cellular_location: CYTOPLASM
gene: hutH
expression_system_strain: BL21 (DE3)
expression_system_cellular_location: CYTOPLASM
expression_system_vector_type: plasmid
expression_system_plasmid: PT7-7H
Authors : Schwede, T.F., Schulz, G.E.
Keywords : AMMONIA-LYASE, HISTIDINE DEGRADATION, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.1 Å )
Citation :

Crystal structure of histidine ammonia-lyase revealing a novel polypeptide modification as the catalytic electrophile.

Schwede, T.F.,Retey, J.,Schulz, G.E.
(1999)  Biochemistry  38 : 5355 - 5361

PubMed: 10220322
DOI: 10.1021/bi982929q

Homogenization and crystallization of histidine ammonia-lyase by exchange of a surface cysteine residue.

Schwede, T.F.,Badeker, M.,Langer, M.  et al.
(1999)  Protein Eng.  12 : 151 - 153

PubMed: 10195286
DOI: 10.1093/protein/12.2.151

Chain : A
UniProt : P21310 (HUTH_PSEPU)
Reaction : L-histidine = urocanate + NH(3).