Brand  (β version)

PDB ID: 1B8F

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
Code Name Show
MDO {2-[(1s)-1-aminoethyl]-4-methylidene-5-oxo-4,5-dihydro-1h-imidazol-1-yl}acetic acid
Glycosylation
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Modification
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Structure summary

Code : 1B8F   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Histidine ammonia-lyase (HAL) from Pseudomonas putida
Release Data : 1999-05-06
Compound :
mol_id molecule chains synonym
1 Histidine ammonia-lyase A HAL, Histidase
ec: 4.3.1.3
mutation: C273A
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:469008)
atcc: 12633
cellular_location: CYTOPLASM
gene: hutH
expression_system_strain: BL21 (DE3)
expression_system_cellular_location: CYTOPLASM
expression_system_vector_type: plasmid
expression_system_plasmid: PT7-7H
Authors : Schwede, T.F., Schulz, G.E.
Keywords : AMMONIA-LYASE, HISTIDINE DEGRADATION, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.1 Å )
Citation :

Crystal structure of histidine ammonia-lyase revealing a novel polypeptide modification as the catalytic electrophile.

Schwede, T.F.,Retey, J.,Schulz, G.E.
(1999)  Biochemistry  38 : 5355 - 5361

PubMed: 10220322
DOI: 10.1021/bi982929q

Homogenization and crystallization of histidine ammonia-lyase by exchange of a surface cysteine residue.

Schwede, T.F.,Badeker, M.,Langer, M.  et al.
(1999)  Protein Eng.  12 : 151 - 153

PubMed: 10195286
DOI: 10.1093/protein/12.2.151

Reaction

Chain : A
UniProt : P21310 (HUTH_PSEPU)
Reaction: EC: Evidence:
Physiological Direction:
L-histidine = NH4(+) + trans-urocanate 4.3.1.3 PubMed:7947813, PubMed:8024588, PubMed:8204579, PubMed:8239649
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