Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
CL Chloride ion
GOL Glycerol
SO4 Sulfate ion
Modified Residues
Code Name Link
CSB Cys bound to lead ion
Code : 1B6G
Header : HYDROLASE
Title : HALOALKANE DEHALOGENASE AT PH 5.0 CONTAINING CHLORIDE
Release Data : 1999-07-13
Compound :
mol_id molecule chains
1 HALOALKANE DEHALOGENASE A
ec: 3.8.1.5
Source :
mol_id organism_scientific expression_system
1 Xanthobacter autotrophicus  (taxid:280) Escherichia coli  (taxid:562)
strain: GJ10
Authors : Ridder, I.S., Rozeboom, H.J., Dijkstra, B.W.
Keywords : HYDROLASE, HALOALKANE DEHALOGENASE, ALPHA/BETA-HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.15 Å )
Citation :

Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 A resolution.

Ridder, I.S.,Rozeboom, H.J.,Dijkstra, B.W.
(1999)  Acta Crystallogr.,Sect.D  55 : 1273 - 1290

PubMed: 10393294
DOI: 10.1107/S090744499900534X

Kinetic Analysis and X-Ray Structure of Haloalkane Dehalogenase with a Modified Halide-Binding Site

Krooshof, G.H.,Ridder, I.S.,Tepper, A.W.  et al.
(1998)  Biochemistry  37 : 15013

Crystallographic Analysis of the Catalytic Mechanism of Haloalkane Dehalogenase

Verschueren, K.H.,Seljee, F.,Rozeboom, H.J.  et al.
(1993)  Nature  363 : 693

Refined X-Ray Structures of Haloalkane Dehalogenase at Ph 6.2 And Ph 8.2 And Implications for the Reaction Mechanism

Verschueren, K.H.,Franken, S.M.,Rozeboom, H.J.  et al.
(1993)  J.Mol.Biol.  232 : 856

Crystal Structure of Haloalkane Dehalogenase: An Enzyme to Detoxify Halogenated Alkanes

Franken, S.M.,Rozeboom, H.J.,Kalk, K.H.  et al.
(1991)  Embo J.  10 : 1297

Crystallization of Haloalkane Dehalogenase from Xanthobacter Autotrophicus Gj10

Rozeboom, H.J.,Kingma, J.,Janssen, D.B.  et al.
(1988)  J.Mol.Biol.  200 : 611

Chain : A
UniProt : P22643 (DHLA_XANAU)
Reaction : 1-haloalkane + H(2)O = a primary alcohol + halide.
1,2-dichloroethane + H(2)O = 2-chloroethanol + hydrogen chloride.