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Ligands
Code Name Style Show Link
CER (2s, 3r)-3-hydroxy-4-oxo-7,10-trans,trans-dodecadienamide
Non-standard Residues
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Glycosylation
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Code : 1B3N   PDBj   RCSB PDB   PDBe
Header : CONDENSING ENZYMES
Title : BETA-KETOACYL CARRIER PROTEIN SYNTHASE AS A DRUG TARGET, IMPLICATIONS FROM THE CRYSTAL STRUCTURE OF A COMPLEX WITH THE INHIBITOR CERULENIN.
Release Data : 1999-04-06
Compound :
mol_id molecule chains
1 PROTEIN (KETOACYL ACYL CARRIER PROTEIN SYNTHASE 2) A
ec: 2.3.1.41
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: FABF
expression_system_strain: M15[PREP4]
expression_system_plasmid: PQE-30
Authors : Moche, M., Schneider, G., Edwards, P., Dehesh, K., Lindqvist, Y.
Keywords : CONDENSING ENZYMES, FATTY ACID ELONGATION, CERULENIN INHIBITION, LIPID METABOLISM, DRUG DESIGN, DRUG TARGET
Exp. method : X-RAY DIFFRACTION ( 2.65 Å )
Citation :

Structure of the complex between the antibiotic cerulenin and its target, beta-ketoacyl-acyl carrier protein synthase.

Moche, M.,Schneider, G.,Edwards, P.  et al.
(1999)  J.Biol.Chem.  274 : 6031 - 6034

PubMed: 10037680
DOI: 10.1074/jbc.274.10.6031

Chain : A
UniProt : P0AAI5 (FABF_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP] - PubMed:22017312, PubMed:9013860
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(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)- octadecenoyl-[ACP] + CO2 + holo-[ACP] 2.3.1.179 PubMed:9013860, PubMed:6988423
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H(+) + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CO2 + holo-[ACP] - PubMed:9013860
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H(+) + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CO2 + holo-[ACP] - PubMed:9013860
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decanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxododecanoyl-[ACP] + CO2 + holo-[ACP] - PubMed:9013860
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dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl- [ACP] + CO2 + holo-[ACP] - PubMed:22017312, PubMed:9013860
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H(+) + malonyl-[ACP] + tetradecanoyl-[ACP] = 3- oxohexadecanoyl-[ACP] + CO2 + holo-[ACP] - PubMed:9013860
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