Brand  (β version)

PDB ID: 1ASW

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
EPE 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid PoSSuM
IPA Isopropyl alcohol PoSSuM
Modified Residues
Code Name Link
MSE Selenomethionine PoSSuM

Structure summary

Code : 1ASW
Header : DNA INTEGRATION
Title : AVIAN SARCOMA VIRUS INTEGRASE CATALYTIC CORE DOMAIN CRYSTALLIZED FROM 20% PEG 4000, 10% ISOPROPANOL, HEPES PH 7.5 USING SELENOMETHIONINE SUBSTITUTED PROTEIN; DATA COLLECTED AT-165 DEGREES C
Release Data : 1995-11-14
Compound :
mol_id molecule chains
1 AVIAN SARCOMA VIRUS INTEGRASE A
mutation: INS(PRO 48, LEU 49, ARG 50, GLU 51, ASN 208, LEU 209)
other_details: CRYSTALLIZED FROM 20% PEG 4000, 10% ISOPROPANOL, HEPES PH 7.5 USING SELENOMETHIONINE-SUBSTITUTED PROTEIN, DATA COLLECTED AT LOW TEMPERATURE
Source :
mol_id organism_scientific expression_system
1 Avian sarcoma virus  (taxid:11876) Escherichia coli  (taxid:562)
strain: SCHMIDT-RUPPIN B
expression_system_plasmid: PRC23IN(52-207)
Authors : Bujacz, G., Jaskolski, M., Alexandratos, J., Wlodawer, A.
Keywords : DNA INTEGRATION
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

High-resolution structure of the catalytic domain of avian sarcoma virus integrase.

Bujacz, G.,Jaskolski, M.,Alexandratos, J.  et al.
(1995)  J.Mol.Biol.  253 : 333 - 346

PubMed: 7563093
DOI: 10.1006/jmbi.1995.0556

Expression, Purification, and Crystallization of Natural and Selenomethionyl Recombinant Ribonuclease H from Escherichia Coli

Yang, W.,Hendrickson, W.A.,Kalman, E.T.  et al.
(1990)  J.Biol.Chem.  265 : 13553

Reaction

Chain : A
UniProt : P03354 (POL_RSVP)
Reaction : Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Endonucleolytic cleavage to 5'- phosphomonoester.