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Code Name Link
HG Mercury (II) ion
Code : 1ARO
Header : complex (polymerase/hydrolase)
Title : t7 RNA polymerase complexed with t7 lysozyme
Release Data : 1998-10-21
Compound :
mol_id 1
molecule t7 rna polymerase
chain P
ec 2.7.7.6
engineered yes
mutation yes
mol_id 2
molecule t7 lysozyme
chain L
synonym n-acetylmuramoyl-l-alanine amidase
ec 3.5.1.28
engineered yes
Source :
mol_id 1
organism_scientific Enterobacteria phage t7
organism_taxid 10760
gene t7
expression_system Escherichia coli bl21
expression_system_taxid 511693
expression_system_strain bl21
expression_system_vector_type t7
expression_system_plasmid t7
expression_system_gene t7
mol_id 2
organism_scientific Enterobacteria phage t7
organism_taxid 10760
gene t7
expression_system Escherichia coli bl21
expression_system_taxid 511693
expression_system_strain bl21
expression_system_vector_type t7
expression_system_plasmid t7
expression_system_gene t7
Authors : T.Steitz, D.Jeruzalmi
Keywords : transcription, dna-directed rna polymerase, hydrolase, glycosidase, complex (polymerase/hydrolase)
Exp. method : X-ray diffraction ( 2.8 Å )
Chain : P
UniProt : P00573 (RPOL_BPT7)
Reaction : Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Chain : L
UniProt : P00806 (ENLYS_BPT7)
Reaction : Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.