Brand  (β version)

PDB ID: 1ARO

Hetero Atom Contents

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hetatm:
centroid:

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Ligands
Code Name Link
HG Mercury (II) ion PoSSuM

Structure summary

Code : 1ARO
Header : COMPLEX (POLYMERASE/HYDROLASE)
Title : T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME
Release Data : 1998-10-21
Compound :
mol_id molecule chains
1 T7 RNA POLYMERASE P
ec: 2.7.7.6
mutation: C347S, C723S, C839S
mol_id molecule chains synonym
2 T7 LYSOZYME L N-ACETYLMURAMOYL-L-ALANINE AMIDASE
ec: 3.5.1.28
Source :
mol_id organism_scientific expression_system
1 Enterobacteria phage T7  (taxid:10760) Escherichia coli BL21  (taxid:511693)
gene: T7
expression_system_strain: BL21
expression_system_vector_type: T7
expression_system_plasmid: T7
expression_system_gene: T7
mol_id organism_scientific expression_system
2 Enterobacteria phage T7  (taxid:10760) Escherichia coli BL21  (taxid:511693)
gene: T7
expression_system_strain: BL21
expression_system_vector_type: T7
expression_system_plasmid: T7
expression_system_gene: T7
Authors : Steitz, T., Jeruzalmi, D.
Keywords : TRANSCRIPTION, DNA-DIRECTED RNA POLYMERASE, HYDROLASE, GLYCOSIDASE, COMPLEX (POLYMERASE-HYDROLASE), COMPLEX (POLYMERASE-HYDROLASE) complex
Exp. method : X-RAY DIFFRACTION ( 2.8 Å )
Citation :

Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme.

Jeruzalmi, D.,Steitz, T.A.
(1998)  EMBO J.  17 : 4101 - 4113

PubMed: 9670025
DOI: 10.1093/emboj/17.14.4101

Reaction

Chain : P
UniProt : P00573 (RPOL_BPT7)
Reaction : Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Chain : L
UniProt : P00806 (ENLYS_BPT7)
Reaction : Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.