Brand  (β version)

PDB ID: 1AL7

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Ligands
Code Name Style Show Link
FMN Flavin mononucleotide PoSSuM
HST 4-carboxy-5-(1-pentyl)hexylsulfanyl-1,2,3-triazole PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1AL7   PDBj   RCSB PDB   PDBe
Header : FLAVOPROTEIN
Title : THREE-DIMENSIONAL STRUCTURES OF GLYCOLATE OXIDASE WITH BOUND ACTIVE-SITE INHIBITORS
Release Data : 1997-11-12
Compound :
mol_id molecule chains
1 GLYCOLATE OXIDASE A
ec: 1.1.3.15
other_details: GLYCOLATE OXIDASE COMPLEXED WITH FMN AND AN INHIBITOR
Source :
mol_id organism_scientific organism_common expression_system
1 Spinacia oleracea  (taxid:3562) Spinach Escherichia coli  (taxid:562)
cell_line: BL21
cellular_location: PEROXISOME
expression_system_strain: BL21 (DE3) PLYSS
expression_system_cellular_location: CYTOPLASM
expression_system_vector_type: PLASMID
expression_system_vector: PLASMID
expression_system_plasmid: PKS20+
Authors : Stenberg, K., Lindqvist, Y.
Keywords : FLAVOPROTEIN, DRUG DESIGN, INHIBITOR BINDING
Exp. method : X-RAY DIFFRACTION ( 2.6 Å )
Citation :

Three-dimensional structures of glycolate oxidase with bound active-site inhibitors.

Stenberg, K.,Lindqvist, Y.
(1997)  Protein Sci.  6 : 1009 - 1015

PubMed: 9144771

High-Level Expression, Purification, and Crystallization of Recombinant Spinach Glycolate Oxidase in Escherichia Coli

Stenberg, K.,Lindqvist, Y.
(1996)  Protein Expr.Purif.  8 : 295

Refined Structure of Spinach Glycolate Oxidase at 2 A Resolution

Lindqvist, Y.
(1989)  J.Mol.Biol.  209 : 151

Reaction

Chain : A
UniProt : P05414 (GOX_SPIOL)
Reaction: EC: Evidence:
Physiological Direction:
glycolate + O2 = glyoxylate + H2O2 1.1.3.15 PubMed:1324737, PubMed:7705356
left-to-right PubMed:7705356
a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2 1.1.3.15 PubMed:7705356
left-to-right