Brand  (β version)

PDB ID: 1AHI

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Ligands
Code Name Style Show Link
CHO Glycochenodeoxycholic acid PoSSuM
NAI 1,4-dihydronicotinamide adenine dinucleotide PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1AHI   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : 7 ALPHA-HYDROXYSTEROID DEHYDROGENASE COMPLEXED WITH NADH AND 7-OXO GLYCOCHENODEOXYCHOLIC ACID
Release Data : 1996-10-14
Compound :
mol_id molecule chains
1 7 ALPHA-HYDROXYSTEROID DEHYDROGENASE A,B
ec: 1.1.1.159
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
strain: HB101
cell_line: HB101
expression_system_strain: DH1
expression_system_plasmid: HB101
Authors : Tanaka, N., Nonaka, T., Mitsui, Y.
Keywords : OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE/REDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.3 Å )
Citation :

Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli.

Tanaka, N.,Nonaka, T.,Tanabe, T.  et al.
(1996)  Biochemistry  35 : 7715 - 7730

PubMed: 8672472
DOI: 10.1021/bi951904d

Crystallization and Preliminary X-Ray Crystallographic Studies of 7Alpha-Hydroxysteroid Dehydrogenase from Escherichia Coli

Tanaka, N.,Nonaka, T.,Yoshimoto, T.  et al.
To be Published 

Cloning and Sequencing of the 7 Alpha-Hydroxysteroid Dehydrogenase Gene from Escherichia Coli Hb101 and Characterization of the Expressed Enzyme

Yoshimoto, T.,Higashi, H.,Kanatani, A.  et al.
(1991)  J.Bacteriol.  173 : 2173

Reaction

Chain : A, B
UniProt : P25529 (HDHA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
cholate + NAD(+) = 3alpha,12alpha-dihydroxy-7-oxo-5beta- cholanate + H(+) + NADH 1.1.1.159 PubMed:2007545, PubMed:9722677
left-to-right PubMed:2007545
chenodeoxycholate + NAD(+) = 7-oxolithocholate + H(+) + NADH - PubMed:2007545
left-to-right
NAD(+) + taurochenodeoxycholate = 7-oxotaurolithocholate + H(+) + NADH - PubMed:2007545
left-to-right
NAD(+) + taurocholate = 7-oxo-taurodeoxycholate + H(+) + NADH - PubMed:2007545
left-to-right
glycocholate + NAD(+) = 7-oxo-glycodeoxycholate + H(+) + NADH - PubMed:2007545
left-to-right
glycochenodeoxycholate + NAD(+) = 7-oxoglycolithocholate + H(+) + NADH - PubMed:8672472
left-to-right