Brand  (β version)

PDB ID: 1AFV

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PB Lead (II) ion PoSSuM
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Structure summary

Code : 1AFV   PDBj   RCSB PDB   PDBe
Header : Viral protein/Immune system
Title : HIV-1 CAPSID PROTEIN (P24) COMPLEX WITH FAB25.3
Release Data : 1997-08-20
Compound :
mol_id molecule chains synonym
1 HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 CAPSID PROTEIN A,B HIV-1 CA, HIV CA, HIV P24, P24
fragment: AMINO-TERMINAL DOMAIN RESIDUES 1 - 151
mol_id molecule chains synonym
2 ANTIBODY FAB25.3 FRAGMENT (LIGHT CHAIN) L,M FAB, FAB LIGHT CHAIN, FAB HEAVY CHAIN
fragment: LIGHT CHAIN RESIDUES 1 - 217, HEAVY CHAIN RESIDUES 1 - 220
mol_id molecule chains synonym
3 ANTIBODY FAB25.3 FRAGMENT (HEAVY CHAIN) H,K FAB, FAB LIGHT CHAIN, FAB HEAVY CHAIN
fragment: LIGHT CHAIN RESIDUES 1 - 217, HEAVY CHAIN RESIDUES 1 - 220
Source :
mol_id organism_scientific expression_system
1 Human immunodeficiency virus 1  (taxid:11676) Escherichia coli BL21(DE3)  (taxid:469008)
strain: C-12
cell_line: BL21
gene: GAG
expression_system_strain: BL21 (DE3)
expression_system_plasmid: PHIV-FSII
expression_system_gene: GAG
other_details: IIIB/LAI ISOLATE OF HIV-1;
mol_id organism_scientific organism_common
2 Mus musculus  (taxid:10090) House mouse
cell_line: BL21
mol_id organism_scientific organism_common
3 Mus musculus  (taxid:10090) House mouse
cell_line: BL21
Authors : Momany, C., Kovari, L.C., Prongay, A.J., Keller, W., Gitti, R.K., Lee, B.M., Gorbalenya, A.E., Tong, L., Mcclure, J., Ehrlich, L.S., Summers, M.F., Carter, C., Rossmann, M.G.
Keywords : COMPLEX (VIRAL CAPSID-IMMUNOGLOBULIN), HIV, CAPSID PROTEIN, P24, Viral protein-Immune system COMPLEX
Exp. method : X-RAY DIFFRACTION ( 3.7 Å )
Citation :

Crystal structure of dimeric HIV-1 capsid protein.

Momany, C.,Kovari, L.C.,Prongay, A.J.  et al.
(1996)  Nat.Struct.Biol.  3 : 763 - 770

PubMed: 8784350
DOI: 10.1038/nsb0996-763

The Use of Antibody Fragments for Crystallization and Structure Determinations

Kovari, L.C.,Momany, C.,Rossmann, M.G.
(1995)  Structure  3 : 1291

Preparation and Crystallization of a Human Immunodeficiency Virus P24-Fab Complex

Prongay, A.J.,Smith, T.J.,Rossmann, M.G.  et al.
(1990)  Proc.Natl.Acad.Sci.USA  87 : 9980

Expression in Escherichia Coli and Purification of Human Immunodeficiency Virus Type 1 Capsid Protein (P24)

Ehrlich, L.S.,Krausslich, H.G.,Wimmer, E.  et al.
(1990)  Aids Res.Hum.Retroviruses  6 : 1169

Reaction

Chain : A, B
UniProt : P12497 (POL_HV1N5)
Reaction: EC: Evidence:
Physiological Direction:
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro. 3.4.23.16 PROSITE-ProRule:PRU00275
-
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. 3.1.26.13 ECO:0000250
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3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. 3.1.13.2 ECO:0000250
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a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.49 PROSITE- ProRule:PRU00405
-
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 PROSITE- ProRule:PRU00405
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Chain : H, K
UniProt : Q99LC4 (Q99LC4_MOUSE)
Reaction : -