Brand  (β version)

PDB ID: 1AFS

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Ligands
Code Name Style Show Link
NAP Nadp nicotinamide-adenine-dinucleotide phosphate PoSSuM
TES Testosterone PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1AFS   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : RECOMBINANT RAT LIVER 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE (3-ALPHA-HSD) COMPLEXED WITH NADP AND TESTOSTERONE
Release Data : 1997-10-08
Compound :
mol_id molecule chains synonym
1 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE A,B 3-ALPHA-HSD
ec: 1.1.1.50
Source :
mol_id organism_scientific organism_common expression_system
1 Rattus norvegicus  (taxid:10116) Norway rat Unidentified  (taxid:32644)
organ: LIVER
Authors : Bennett, M.J., Albert, R.H., Jez, J.M., Ma, H., Penning, T.M., Lewis, M.
Keywords : OXIDOREDUCTASE, NAD
Exp. method : X-RAY DIFFRACTION ( 2.5 Å )
Citation :

Steroid recognition and regulation of hormone action: crystal structure of testosterone and NADP+ bound to 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase.

Bennett, M.J.,Albert, R.H.,Jez, J.M.  et al.
(1997)  Structure  5 : 799 - 812

PubMed: 9261071
DOI: 10.1016/S0969-2126(97)00234-7

Structure of 3 Alpha-Hydroxysteroid/Dihydrodiol Dehydrogenase Complexed with Nadp+

Bennett, M.J.,Schlegel, B.P.,Jez, J.M.  et al.
(1996)  Biochemistry  35 : 10702

Three-Dimensional Structure of Rat Liver 3 Alpha-Hydroxysteroid/Dihydrodiol Dehydrogenase: A Member of the Aldo-Keto Reductase Superfamily

Hoog, S.S.,Pawlowski, J.E.,Alzari, P.M.  et al.
(1994)  Proc.Natl.Acad.Sci.USA  91 : 2517

Reaction

Chain : A, B
UniProt : P23457 (DIDH_RAT)
Reaction: EC: Evidence:
Physiological Direction:
a 3alpha-hydroxysteroid + NADP(+) = a 3-oxosteroid + H(+) + NADPH 1.1.1.50 -
-
a 3alpha-hydroxysteroid + NAD(+) = a 3-oxosteroid + H(+) + NADH 1.1.1.50 -
-