Brand  (β version)

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Ligands
Code Name Link
FMT Formic acid
NI Nickel (II) ion
Code : 1A5O
Header : HYDROLASE
Title : K217C VARIANT OF KLEBSIELLA AEROGENES UREASE, CHEMICALLY RESCUED BY FORMATE AND NICKEL
Release Data : 1998-05-27
Compound :
mol_id molecule chains
1 UREASE (GAMMA SUBUNIT) A
ec: 3.5.1.5
mutation: K217C, C319A
mol_id molecule chains
2 UREASE (BETA SUBUNIT) B
ec: 3.5.1.5
mol_id molecule chains
3 UREASE (ALPHA SUBUNIT) C
ec: 3.5.1.5
Source :
mol_id organism_scientific expression_system
1 Klebsiella aerogenes  (taxid:28451) Escherichia coli  (taxid:562)
gene: UREA, UREB, UREC
expression_system_strain: DH5
expression_system_plasmid: PKAU17
expression_system_gene: UREA, UREB, UREC
mol_id organism_scientific expression_system
2 Klebsiella aerogenes  (taxid:28451) Escherichia coli  (taxid:562)
gene: UREA, UREB, UREC
expression_system_strain: DH5
expression_system_plasmid: PKAU17
expression_system_gene: UREA, UREB, UREC
mol_id organism_scientific expression_system
3 Klebsiella aerogenes  (taxid:28451) Escherichia coli  (taxid:562)
gene: UREA, UREB, UREC
expression_system_strain: DH5
expression_system_plasmid: PKAU17
expression_system_gene: UREA, UREB, UREC
Authors : Pearson, M.A., Schaller, R.A., Michel, L.O., Karplus, P.A., Hausinger, R.P.
Keywords : HYDROLASE (UREA AMIDO), MUTANT, NICKEL METALLOENZYME, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.5 Å )
Citation :

Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand.

Pearson, M.A.,Schaller, R.A.,Michel, L.O.  et al.
(1998)  Biochemistry  37 : 6214 - 6220

PubMed: 9558361
DOI: 10.1021/bi980021u

70 Years of Crystalline Urease: What Have We Learned?

Karplus, P.A.,Pearson, M.A.,Hausinger, R.P.
(1997)  Acc.Chem.Res.  30 : 330

The Crystal Structure of Urease from Klebsiella Aerogenes

Jabri, E.,Carr, M.B.,Hausinger, R.P.  et al.
(1995)  Science  268 : 998

Chain : A
UniProt : P18316 (URE3_ENTAE)
Reaction : Urea + H(2)O = CO(2) + 2 NH(3).
Chain : B
UniProt : P18315 (URE2_ENTAE)
Reaction : Urea + H(2)O = CO(2) + 2 NH(3).
Chain : C
UniProt : P18314 (URE1_ENTAE)
Reaction : Urea + H(2)O = CO(2) + 2 NH(3).