Brand  (β version)

PDB ID: 1A5N

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
FMT Formic acid PoSSuM
NI Nickel (II) ion PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1A5N   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : K217A VARIANT OF KLEBSIELLA AEROGENES UREASE, CHEMICALLY RESCUED BY FORMATE AND NICKEL
Release Data : 1998-05-27
Compound :
mol_id molecule chains
1 UREASE (GAMMA SUBUNIT) A
ec: 3.5.1.5
mutation: K217A
mol_id molecule chains
2 UREASE (BETA SUBUNIT) B
ec: 3.5.1.5
mol_id molecule chains
3 UREASE (ALPHA SUBUNIT) C
ec: 3.5.1.5
Source :
mol_id organism_scientific expression_system
1 Klebsiella aerogenes  (taxid:28451) Escherichia coli  (taxid:562)
gene: UREA, UREB, UREC
expression_system_strain: DH5
expression_system_plasmid: PKAU17
expression_system_gene: UREA, UREB, UREC
mol_id organism_scientific expression_system
2 Klebsiella aerogenes  (taxid:28451) Escherichia coli  (taxid:562)
gene: UREA, UREB, UREC
expression_system_strain: DH5
expression_system_plasmid: PKAU17
expression_system_gene: UREA, UREB, UREC
mol_id organism_scientific expression_system
3 Klebsiella aerogenes  (taxid:28451) Escherichia coli  (taxid:562)
gene: UREA, UREB, UREC
expression_system_strain: DH5
expression_system_plasmid: PKAU17
expression_system_gene: UREA, UREB, UREC
Authors : Pearson, M.A., Schaller, R.A., Michel, L.O., Karplus, P.A., Hausinger, R.P.
Keywords : HYDROLASE (UREA AMIDO), MUTANT, NICKEL METALLOENZYME, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.4 Å )
Citation :

Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand.

Pearson, M.A.,Schaller, R.A.,Michel, L.O.  et al.
(1998)  Biochemistry  37 : 6214 - 6220

PubMed: 9558361
DOI: 10.1021/bi980021u

70 Years of Crystalline Urease: What Have We Learned?

Karplus, P.A.,Pearson, M.A.,Hausinger, R.P.
(1997)  Acc.Chem.Res.  30 : 330

The Crystal Structure of Urease from Klebsiella Aerogenes

Jabri, E.,Carr, M.B.,Hausinger, R.P.  et al.
(1995)  Science  268 : 998

Reaction

Chain : A
UniProt : P18316 (URE3_KLEAE)
Reaction: EC: Evidence:
Physiological Direction:
2 H(+) + H2O + urea = CO2 + 2 NH4(+) 3.5.1.5 HAMAP-Rule:MF_00739, PubMed:10500143, PubMed:11015224, PubMed:1400317, PubMed:2211515, PubMed:7721685, PubMed:7855593, PubMed:7909161, PubMed:8318888, PubMed:8808930
-
Chain : B
UniProt : P18315 (URE2_KLEAE)
Reaction: EC: Evidence:
Physiological Direction:
2 H(+) + H2O + urea = CO2 + 2 NH4(+) 3.5.1.5 HAMAP-Rule:MF_01954, PubMed:10500143, PubMed:11015224, PubMed:1400317, PubMed:2211515, PubMed:7721685, PubMed:7855593, PubMed:7909161, PubMed:8318888, PubMed:8808930
-
Chain : C
UniProt : P18314 (URE1_KLEAE)
Reaction: EC: Evidence:
Physiological Direction:
2 H(+) + H2O + urea = CO2 + 2 NH4(+) 3.5.1.5 HAMAP-Rule:MF_01953, PubMed:10500143, PubMed:11015224, PubMed:1400317, PubMed:2211515, PubMed:7721685, PubMed:7855593, PubMed:7909161, PubMed:8318888, PubMed:8808930
-