Brand  (β version)

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Ligands
Code Name Link
K Potassium ion
IGP Indole-3-glycerol phosphate
PLP Pyridoxal-5'-phosphate
Code : 1A5B
Header : LYASE
Title : CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHA D60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49
Release Data : 1998-05-27
Compound :
mol_id molecule chains
1 TRYPTOPHAN SYNTHASE (ALPHA CHAIN) A
ec: 4.2.1.20
mutation: D60N
other_details: LIGAND INDOLE-3-GLYCEROL PHOSPHATE BOUND TO THE A-SUBUNIT
mol_id molecule chains
2 TRYPTOPHAN SYNTHASE (BETA CHAIN) B
ec: 4.2.1.20
other_details: LIGAND INDOLE-3-GLYCEROL PHOSPHATE BOUND TO THE A-SUBUNIT
Source :
mol_id organism_scientific expression_system
1 Salmonella typhimurium  (taxid:602) Escherichia coli  (taxid:562)
cell_line: CB149
gene: TRPA/TRPB
expression_system_cell_line: CB149
expression_system_plasmid: PSTB7
expression_system_gene: TRPA/TRPB
mol_id organism_scientific expression_system
2 Salmonella typhimurium  (taxid:602) Escherichia coli  (taxid:562)
cell_line: CB149
gene: TRPA/TRPB
expression_system_cell_line: CB149
expression_system_plasmid: PSTB7
expression_system_gene: TRPA/TRPB
Authors : Rhee, S., Miles, E.W., Davies, D.R.
Keywords : CARBON-OXYGEN LYASE, MUTATION AT POSITION 60 (ASP --> ASN) IN THE A-SUBUNIT, TRUE SUBSTRATE INDOLE-3-GLYCEROL PHOSPHATE IN THE A-SUBUNIT, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.0 Å )
Citation :

Cryo-crystallography of a true substrate, indole-3-glycerol phosphate, bound to a mutant (alphaD60N) tryptophan synthase alpha2beta2 complex reveals the correct orientation of active site alphaGlu49.

Rhee, S.,Miles, E.W.,Davies, D.R.
(1998)  J.Biol.Chem.  273 : 8553 - 8555

PubMed: 9535826
DOI: 10.1074/jbc.273.15.8553

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction : L-serine + 1-C-(indol-3-yl)glycerol 3- phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H(2)O.
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction : L-serine + 1-C-(indol-3-yl)glycerol 3- phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H(2)O.