Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
PO4 Phosphate ion
Code : 1A40
Header : PHOSPHATE-BINDING PROTEIN
Title : PHOSPHATE-BINDING PROTEIN WITH ALA 197 REPLACED WITH TRP
Release Data : 1999-03-23
Compound :
mol_id molecule chains synonym
1 PHOSPHATE-BINDING PERIPLASMIC PROTEIN PRECURSOR A PBP A197W
mutation: A197W
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
strain: PAN92
expression_system_strain: PET-22B
expression_system_vector_type: PLASMID
Authors : Ledivina, P.S., Wang, Z., Tsai, A., Koehl, E., Quiocho, F.A.
Keywords : PHOSPHATE-BINDING PROTEIN, MUTAGENESIS, CHARGE COMPLEMENTARY, KINETICS
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Dominant role of local dipolar interactions in phosphate binding to a receptor cleft with an electronegative charge surface: equilibrium, kinetic, and crystallographic studies.

Ledvina, P.S.,Tsai, A.L.,Wang, Z.  et al.
(1998)  Protein Sci.  7 : 2550 - 2559

PubMed: 9865949

A Low Energy Short Hydrogen Bond in Very High Resolution Structures of Protein Receptor--Phosphate Complexes

Wang, Z.,Luecke, H.,Yao, N.  et al.
(1997)  Nat.Struct.Biol.  4 : 519

Negative Electrostatic Surface Potential of Protein Sites Specific for Anionic Ligands

Ledvina, P.S.,Yao, N.,Choudhary, A.  et al.
(1996)  Proc.Natl.Acad.Sci.USA  93 : 6786

Fine Tuning the Specificity of the Periplasmic Phosphate Transport Receptor. Site-Directed Mutagenesis, Ligand Binding, and Crystallographic Studies

Wang, Z.,Choudhary, A.,Ledvina, P.S.  et al.
(1994)  J.Biol.Chem.  269 : 25091

Chain : A
UniProt : P06128 (multiple entry names are found)
Reaction : -