Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
CA Calcium ion
THP Thymidine-3',5'-diphosphate
Modified Residues
Code Name Link
CME S,S-(2-hydroxyethyl)thiocysteine
Code : 1A2T   PDBj   RCSB PDB   PDBe
Header : NUCLEASE
Title : STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT
Release Data : 1998-04-29
Compound :
mol_id molecule chains
1 STAPHYLOCOCCAL NUCLEASE A
ec: 3.1.31.1
mutation: V23C, B-MERCAPTOETHANOL DISULFIDE
other_details: VARIANT FORMED BY CHEMICAL MODIFICATION OF SOLE CYSTEINE RESIDUE
Source :
mol_id organism_scientific expression_system
1 Staphylococcus aureus  (taxid:1280) Escherichia coli  (taxid:562)
expression_system_strain: AR120
expression_system_vector: PAS1
Authors : Wynn, R., Harkins, P.C., Richards, F.M., Fox, R.O.
Keywords : NUCLEASE, UNNATURAL AMINO ACID, HYDROLASE, ENDONUCLEASE
Exp. method : X-RAY DIFFRACTION ( 1.96 Å )
Citation :

Mobile unnatural amino acid side chains in the core of staphylococcal nuclease.

Wynn, R.,Harkins, P.C.,Richards, F.M.  et al.
(1996)  Protein Sci.  5 : 1026 - 1031

PubMed: 8762134

Interactions in Nonnative and Truncated Forms of Staphylococcal Nuclease as Indicated by Mutational Free Energy Changes

Wynn, R.,Anderson, C.L.,Richards, F.M.  et al.
(1995)  Protein Sci.  4 : 1815

Unnatural Amino Acid Packing Mutants of Escherichia Coli Thioredoxin Produced by Combined Mutagenesis/Chemical Modification Techniques

Wynn, R.,Richards, F.M.
(1993)  Protein Sci.  2 : 395

Chain : A
UniProt : P00644 (NUC_STAAU)
Reaction : Endonucleolytic cleavage to nucleoside 3'- phosphates and 3'-phosphooligonucleotide end-products.