Brand  (β version)

PDB ID: 1A2T

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
THP Thymidine-3',5'-diphosphate PoSSuM
Non-standard Residues
Code Name Show
CME S,S-(2-hydroxyethyl)thiocysteine
Glycosylation
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Modification
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Structure summary

Code : 1A2T   PDBj   RCSB PDB   PDBe
Header : NUCLEASE
Title : STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT
Release Data : 1998-04-29
Compound :
mol_id molecule chains
1 STAPHYLOCOCCAL NUCLEASE A
ec: 3.1.31.1
mutation: V23C, B-MERCAPTOETHANOL DISULFIDE
other_details: VARIANT FORMED BY CHEMICAL MODIFICATION OF SOLE CYSTEINE RESIDUE
Source :
mol_id organism_scientific expression_system
1 Staphylococcus aureus  (taxid:1280) Escherichia coli  (taxid:562)
expression_system_strain: AR120
expression_system_vector: PAS1
Authors : Wynn, R., Harkins, P.C., Richards, F.M., Fox, R.O.
Keywords : NUCLEASE, UNNATURAL AMINO ACID, HYDROLASE, ENDONUCLEASE
Exp. method : X-RAY DIFFRACTION ( 1.96 Å )
Citation :

Mobile unnatural amino acid side chains in the core of staphylococcal nuclease.

Wynn, R.,Harkins, P.C.,Richards, F.M.  et al.
(1996)  Protein Sci.  5 : 1026 - 1031

PubMed: 8762134

Interactions in Nonnative and Truncated Forms of Staphylococcal Nuclease as Indicated by Mutational Free Energy Changes

Wynn, R.,Anderson, C.L.,Richards, F.M.  et al.
(1995)  Protein Sci.  4 : 1815

Unnatural Amino Acid Packing Mutants of Escherichia Coli Thioredoxin Produced by Combined Mutagenesis/Chemical Modification Techniques

Wynn, R.,Richards, F.M.
(1993)  Protein Sci.  2 : 395

Reaction

Chain : A
UniProt : P00644 (NUC_STAAU)
Reaction: EC: Evidence:
Physiological Direction:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'- phosphooligonucleotide end-products. 3.1.31.1 PROSITE-ProRule:PRU10048, PROSITE-ProRule:PRU10049
-
Cofactor: Evidence: Note:
Ca(2+) - Binds 1 Ca(2+) ion per subunit.