Brand  (β version)

PDB ID: 1A1V

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
Non-standard Residues
Code Name Show
CME S,S-(2-hydroxyethyl)thiocysteine
Glycosylation
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Structure summary

Code : 1A1V   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/DNA
Title : HEPATITIS C VIRUS NS3 HELICASE DOMAIN COMPLEXED WITH SINGLE STRANDED SDNA
Release Data : 1999-01-13
Compound :
mol_id molecule chains
1 DNA (5'-D(*UP*UP*UP*UP*UP*UP*UP*U)-3') B
other_details: SINGLE STRANDED DNA
mol_id molecule chains
2 PROTEIN (NS3 PROTEIN) A
fragment: HELICASE DOMAIN
mutation: N-TERMINAL MET, K221Q, A277G, S301L, S332P, S410A, G530E, R582W, AND A 10 RESIDUE (GSGSHHHHHH) HISTIDINE TAG ATTACHED TO THE C-TERMINUS
Source :
mol_id organism_scientific
1
synthetic: yes
other_details: SYNTHETIC DEOXYOLIGONUCLEOTIDE
mol_id organism_scientific expression_system
2 Hepatitis C virus (isolate H)  (taxid:11108) Escherichia coli  (taxid:562)
strain: H
gene: NS3
expression_system_strain: JM109
expression_system_cellular_location: CYTOPLASM
expression_system_plasmid: PET-BS(+)
Authors : Kim, J.L., Morgenstern, K.A., Griffith, J.P., Dwyer, M.D., Thomson, J.A., Murcko, M.A., Lin, C., Caron, P.R.
Keywords : HEPATITIS C VIRUS, RNA HELICASE, NONSTRUCTURAL PROTEINS, SINGLE-STRANDED DNA, HYDROLASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.2 Å )
Citation :

Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding.

Kim, J.L.,Morgenstern, K.A.,Griffith, J.P.  et al.
(1998)  Structure  6 : 89 - 100

PubMed: 9493270
DOI: 10.1016/S0969-2126(98)00010-0

Reaction

Chain : A
UniProt : P27958 (POLG_HCV77)
Reaction: EC: Evidence:
Physiological Direction:
[Serine protease/helicase NS3]
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'. 		3.4.21.98 PubMed:8035505, PubMed:8386278
-
[Serine protease/helicase NS3]
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'- diphosphate + H(+) + phosphate 		3.6.1.15 PubMed:15269774, PubMed:16397502, PubMed:25551442
-
[Serine protease/helicase NS3]
ATP + H2O = ADP + H(+) + phosphate 		3.6.4.13 PubMed:15269774, PubMed:16397502, PubMed:25551442
-
[RNA-directed RNA polymerase]
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1) 		2.7.7.48 PROSITE-ProRule:PRU00539, PubMed:20729191
-