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Ligands
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ATP Adenosine-5'-triphosphate
Non-standard Residues
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Glycosylation
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Code : 1A0I   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7 COMPLEX WITH ATP
Release Data : 1998-03-25
Compound :
mol_id molecule chains
1 DNA LIGASE A
ec: 6.5.1.1
mutation: M2V
Source :
mol_id organism_scientific expression_system
1 Enterobacteria phage T7  (taxid:10760) Escherichia coli  (taxid:562)
gene: LIG
expression_system_strain: B834
expression_system_vector_type: T7
expression_system_vector: PET22
expression_system_plasmid: T7
expression_system_gene: T7
Authors : Subramanya, H.S., Doherty, A.J., Ashford, S.R., Wigley, D.B.
Keywords : LIGASE, DNA REPLICATION
Exp. method : X-RAY DIFFRACTION ( 2.6 Å )
Citation :

Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7.

Subramanya, H.S.,Doherty, A.J.,Ashford, S.R.  et al.
(1996)  Cell(Cambridge,Mass.)  85 : 607 - 615

PubMed: 8653795
DOI: 10.1016/S0092-8674(00)81260-X

Chain : A
UniProt : P00969 (DNLI_BPT7)
Reaction: EC: Evidence:
Physiological Direction:
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate. 6.5.1.1 PROSITE- ProRule:PRU10135, PubMed:8653795
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