Brand  (β version)

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centroid:
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Ligands
Code Name Link
BME Beta-mercaptoethanol
MUB N-acetylmuramic acid
NAG N-acetyl-D-glucosamine
Modified Residues
Code Name Link
API 2,6-diaminopimelic acid
DAL D-alanine
FGA Gamma-D-glutamic acid
Code : 148L
Header : HYDROLASE/HYDROLASE SUBSTRATE
Title : A COVALENT ENZYME-SUBSTRATE INTERMEDIATE WITH SACCHARIDE DISTORTION IN A MUTANT T4 LYSOZYME
Release Data : 1994-04-30
Compound :
mol_id molecule chains
1 T4 LYSOZYME E
ec: 3.2.1.17
mol_id molecule chains
2 SUBSTRATE CLEAVED FROM CELL WALL OF ESCHERICHIA COLI S
Source :
mol_id organism_scientific
1 Enterobacteria phage T4  (taxid:10665)
expression_system_vector_type: PLASMID
expression_system_plasmid: M13
mol_id organism_scientific
2 ESCHERICHIA COLI  (taxid:562)
tissue: CELL WALL
Authors : Kuroki, R., Weaver, L.H., Matthews, B.W.
Keywords : O-GLYCOSYL, HYDROLASE-HYDROLASE SUBSTRATE complex
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

A covalent enzyme-substrate intermediate with saccharide distortion in a mutant T4 lysozyme.

Kuroki, R.,Weaver, L.H.,Matthews, B.W.
(1993)  Science  262 : 2030 - 2033

PubMed: 8266098

Structure of Bacteriophage T4 Lysozyme Refined at 1.7 Angstroms Resolution

Weaver, L.H.,Matthews, B.W.
(1987)  J.Mol.Biol.  193 : 189

Chain : E
UniProt : P00720 (ENLYS_BPT4)
Reaction : Hydrolysis of (1->4)-beta-linkages between N- acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.